Journal: Cells
Article Title: Cell-Specific Effects of Insulin in a Murine Model of Restenosis Under Insulin-Sensitive and Insulin-Resistant Conditions
doi: 10.3390/cells13161387
Figure Lengend Snippet: Metabolic parameters of tamoxifen-treated endothelial-cell-specific IR-deficient (IR f/f -Cdh5-Cre (+)) and control (IR f/f -Cdh5-Cre (−)) mice. LFD: Low-fat diet; HFSD: High-fat, high-sucrose diet. Data are expressed as mean ± SEM. Parametric or non-parametric tests were applied according to the results of normality tests. Three-way ANOVA followed by Tukey’s test: significant diet main effect was found for body weight, fasting plasma glucose, fed plasma glucose, IGF-1, triglycerides, free fatty acids, and total cholesterol (* p < 0.05); significant treatment main effect was found for insulin, triglycerides, and free fatty acids ( $ p < 0.001). n > 6 for each group.
Article Snippet: In addition, blood samples were collected via cardiac puncture at the time of sacrifice for analysis of metabolic parameters, such as plasma insulin (ELISA obtained from ALPCO, Salem, NH, USA, Catalog #80-INSMS-E01, E10), insulin-like growth factor 1 (IGF-1) (ELISA obtained from R&D systems, Minneapolis, MN, USA, Catalog #MG100), vascular endothelial growth factor (VEGF) (ELISA obtained from R&D systems, Minneapolis, MN, USA, Catalog #MMV00-1), triglyceride (TG), free fatty acids (FFAs) (both colorimetric assay technique, Wako Diagnostics, Osaka, Japan), and total cholesterol (Infinity Cholesterol Kit obtained from Thermo Scientific, Waltham, MA, USA, Catalog #TR13421).
Techniques: Control, Clinical Proteomics